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KMID : 1007520150240031147
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Food Science and Biotechnology
2015 Volume.24 No. 3 p.1147 ~ p.1150
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β-Glucan from Aureobasidum Species Inhibits Fat Accumulation in 3T3-L1 Adipocyte Differentiation
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Kim Mi-Ja
Kim Ork-Hee
Cheong Chul
Jang Ki-Hyo
Kim Chul-Ho
Kang Soon-Ah
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Abstract
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The anti-fat accumulative effects of bacteriaderived β-glucan in 3T3-L1 adipocyte differentiation was investigated. 3T3-L1 preadipocytes were treated with β- glucan at 50 and 100 μg/mL during adipogenesis. Oil Red O staining was used to study expressions of the adipogenic genes PPARγ, ACC, and aP2. Adipogenesis of 3T3-L1 cells treated with β-glucan at 50 and 100 μg/mL was significantly (p<0.05) inhibited by 32.8 and 39.7%, respectively, compared with controls. Expressions of the PPARγ gene from 3T3-L1 cells treated with β-glucan at 50 and 100 μg/mL were significantly (p<0.05) decreased by 13.6 and 46.1%, respectively, ACC gene expression by 18.3 and 36.0%, respectively, and aP2 gene expression by 36.8 and 56.7%, respectively, compared to adipocyte controls. Bacteria-derived β-glucan inhibits fat accumulation at the cellular level that is mediated via decreases in expressions of the PPARγ, ACC, and aP2 genes.
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KEYWORD
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acterial derived β-glucan, 3T3-L1, proliferatoractivated receptor γ, acetyl CoA carboxylase, adipocyte lipid-binding protein
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